Thanks for sharing your work!
I have a couple of questions: what’s the Glucose concentration that you used for low glucose? Do you see time-dependent adaptations to low glucose? I mean, how long after putting your cells in low glucose did you see changes in gene expression? Are this maintained if you keep the cells in low glucose or are they only an acute response? Finally, have you look whether there is any difference in glucose dependency in cells grown in 3D compared to 2D?
Thanks a lot